The Short Answer: Only when the decal is printed on translucent vinyl. Standard printed vinyl blocks light.
Yes, but only with light transmitting vinyl decals. Standard printed vinyl decals are opaque and block light; a decal printed on translucent vinyl lets light pass through and spreads it evenly, so the graphic glows when it's lit from behind. Decals.com offers two light transmitting materials: translucent adhesive vinyl, which sticks to glass, acrylic sign faces, and lighted displays, and translucent backlit film, a non-adhesive print made for lightbox inserts.
Opaque custom decals are designed to show color and detail under normal lighting, which is what most car, window, and equipment graphics need. If you want a decal that glows from behind, such as a backlit sign panel, an illuminated window graphic, or a lit-up storefront logo, you need a material engineered for it. Translucent vinyl uses a light-diffusing base film so the printed image glows evenly instead of showing dark silhouettes or harsh hot spots. Decals.com prints and ships these materials direct to you as finished, ready-to-apply decals, cut and sized to your artwork.